The influence of biologically active preparations on the preservation of boar semen

Abstract

The research was conducted on sperm collected from breeding males. Collection was performed using the manual method. For processing, ejaculates with a motility of no less than 70% and a concentration of 0.25 billion per milliliter were accepted. The study utilized the biologically active preparation MP extracted from cyanobacterial yeasts. The biologically active preparation IMB-2 was introduced as an additional component in the GHTS medium at concentrations ranging from 0.2% to 1.2%. The experimental results allowed for the attainment of sperm motility after 120 hours of storage at hypothermic temperatures, measuring 60.3±2.3%, with morphological indices at 63.0±0.7% and a total anomaly rate of 7.8% when the concentration of IMB-2 was added as an additional component at 0.7% in the base medium. In comparison, the control group showed these indices at 50.3±2.9%, 53.5±1.2%, and 10.2%, respectively. The average sperm progression speed after 120 hours was as follows: VAP (velocity average path) - 59.6±5.1%; VSL (velocity straight line) - 28.3±2.4%; and VCL (velocity curve line) - 115.4±1.9%.