Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes

Abstract

To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic enzymes metal oxide nanoparticles: MgO, ZnO, ZnO/MgO in a ratio of 1:4, TiO2 and Fe3O4 through hydrothermal and sol-gel techniques were synthesized. These nanomaterials were characterized by powder X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR) and SEM-microscopy. The assessment of the influence of synthesized nano metal oxides, was carried out according to their impact on biosynthesis of the enzyme at micromycetes Trichoderma koningii, Fusarium gibbosum and Aspergillus niger – producers of extracellular protease and amylase. It was found that the impact of nanomaterials on the synthesis of hydrolases depends on the structure, dimension and concentration of the nanoparticles used as well as on the physiological and biochemical characteristics of the strain and the specificity of action of the enzymes synthesized by them.