Acetic acid bacteria detection in wines by real-time PCR

ZGARDAN, Dan; MITINA, Irina; MITIN, Valentin; BEHTA, Emilia BEHTA; RUBTOV, Silvia; BOISTEAN, Alina; STURZA, Rodica; MUNTEANU, Maria

dc.contributor.author	ZGARDAN, Dan
dc.contributor.author	MITINA, Irina
dc.contributor.author	MITIN, Valentin
dc.contributor.author	BEHTA, Emilia BEHTA
dc.contributor.author	RUBTOV, Silvia
dc.contributor.author	BOISTEAN, Alina
dc.contributor.author	STURZA, Rodica
dc.contributor.author	MUNTEANU, Maria
dc.date.accessioned	2024-05-15T08:59:00Z
dc.date.available	2024-05-15T08:59:00Z
dc.date.issued	2022
dc.identifier.citation	ZGARDAN, Dan et al. Acetic acid bacteria detection in wines by real-time PCR. In: Studii şi Cercetări Ştiinţifice. Chimie şi Inginerie Chimică, Biotehnologii, Industrie Alimentară = Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2022. vol. 23, nr. 2, pp. 179-188. ISSN 1582-540X.	en_US
dc.identifier.issn	1582-540X
dc.identifier.uri	http://repository.utm.md/handle/5014/27090
dc.description.abstract	Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RTPCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.	en_US
dc.language.iso	en	en_US
dc.publisher	University of Bacău	en_US
dc.relation.ispartofseries	Studii şi Cercetări Ştiinţifice. Chimie şi Inginerie Chimică, Biotehnologii, Industrie Alimentară = Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry;2022. vol. 23, nr. 2
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 United States	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.subject	acetic acid bacteria	en_US
dc.subject	wine spoilage	en_US
dc.subject	microorganisms	en_US
dc.subject	polymerase chain reaction	en_US
dc.subject	primers	en_US
dc.title	Acetic acid bacteria detection in wines by real-time PCR	en_US
dc.type	Article	en_US