Technological potential and changes in bioactive compounds during ripening of four sea Buckthorn (Hippophae rhamnoides L.) cultivars grown in the Republic of Moldova

Abstract

Sea buckthorn (Hippophae rhamnoides L.), known as a “superfruit” due to its complex nutritional profile, exhibits significant variations in bioactive compounds depending on variety and ripening stage. This study evaluates the ripening dynamics of four specific varieties-Dora, Cora, Clara, and Mara-cultivated in the Republic of Moldova to identify the optimal harvest window and technological potential for each variety. Over a sevenweek period (August-September), several physicochemical and biochemical parameters were monitored, including pH, titratable acidity, total soluble solids (TSS), total dry matter (TDM), lipid content, color, hardness, vitamin C, total carotenoids content (TCC), and organic acids. Analytical methods such as refractometry, spectrophotometry, and capillary electrophoresis were employed to track these components. A downward trend in TSS and titratable acidity, characteristic of non-climacteric fruits, was observed. The Cora and Mara varieties exhibited the highest productivity and total dry matter content (up to 35.61%), making them suitable for concentrated food products. Clara stood out with the highest lipid accumulation (reaching 2.32%), identifying it as the premium variety for oil extraction. Vitamin C levels generally decreased across all varieties during ripening, with the Mara variety maintaining the highest concentrations (up to 518 mg/100 g). Conversely, total carotenoids showed a peak during full maturity (late August) for most varieties, with Dora reaching the highest values (40.73 mg/100 g). The present study highlights that the optimal harvest time for maximizing bioactive compounds is between late August and early September.