The influence of a complex biologically active preparation on the preservation of the reproductive potential of the sperm of stud rams after cryopreservation

Abstract

In sheep farming, the adoption of reproductive technologies remains at a low level. One of the key challenges is the reduced efficiency of using frozen semen from stud rams. This is due to the fact that cryopreservation and subsequent thawing processes result in significant cell losses and structural damage, which greatly affect the quality of the material. To develop a method for long-term storage and preservation of the biological value of spermatozoa during the process of dilution, freezing and thawing of ram sperm, a liquid biologically active microbial preparation (MPSP) containing yeast manoprotein and sulfated cyanobacterial polysaccharides with antioxidant action, introduced into the composition of the synthetic sucrose-citrate-egg yolk (SȚJ) medium in different concentrations, was tested. It was found that the introduction of the drug MPSP into the SȚJ medium at concentrations of 0.4-0.8%/V allowed us to obtain the highest indicators of motility, survival, preservation of acrosome integrity, and speed of sperm movement compared to the control group.