Process for obtaining the biomass of the red microalga Porphyridium cruentum – source of omega 3 lipids with polyvalent properties

Abstract

The invention relates to a proceeding for cultivating the red microalga of biotechnological interest Porphyridium cruentum, in order to obtain biomass with a high omega-3 lipid content. The proceeding involves the cultivation of microalga Porphyridium cruentum CNMN-AR-01 on a nutrient medium containing citrate-stabilized gold nanoparticles 5 nm in size, in the concentration range of 4.8 - 5.1 nM, for 14 days at a constant temperature of 25-280C and continuous illumination with an intensity of 50-57 μmol photons/m2 and periodic slow stirring. The result of the invention consists in increasing the lipid content of algae biomass by about 52%. This result is due to the use of gold nanoparticles 5 nm diameter as a stimulator of lipid biosynthesis by the marine microalga Porphyridium cruentum, a valuable producer of omega-3 lipids. Porphyridium biomass obtained according to this proceeding contain biofunctionalized gold nanoparticles and can be used as a raw material for the manufacture and development of new nutraceuticals and original remedies based on omega-3 lipids and biofunctionalized gold nanoparticles with antioxidant, anti-inflammatory, antiatherogenic and regenerative properties. The research was carried out within the project 20.80009.5007.05 "Biofunctionalized metal nanoparticles –obtaining using cyanobacteria and microalgae", funded by NARD, Republic of Moldova.