New method for obtaining the proteoglycan preparation

Abstract

The invention relates to the elaboration of a new process for obtaining of proteoglycan preparation with high content of sulfated polysaccharides from the remaining biomass of Arthrospira platensis from the production of remedy BioR. Using biomass of cyanobacteria Arthrospira platensis as the source of proteoglycans which possess anti-inflammatory, antiviral activities for the application in zootechny and other fields. The process according to the invention consists in the following steps: the dried at the temperature of +50±5°C remaining biomass Arthrospira platensis was subjected to grinding, then it was mixed with 96% ethyl alcohol in a volume of 1:10, the obtained suspension was placed in a water bath at a temperature of +45˚C, for 30 minutes, centrifugation at 3500 rpm., the sediment was mixed with distilled water at a ratio of 1:3 v/v and placed under 50 W ultrasound for 5 minutes and heated at a temperature of +45˚C for 30 minutes or was mixed with distilled water at a ratio of 1:3 v/v and subjected to autoclaving at a temperature of +115° C (0.5 atm.) for 30 minutes, centrifugation at 3500 rpm. and add EDTA to the final proteoglycan preparation. The technical result of the invention consists in the elaboration of the procedure for obtaining the proteoglycan preparation with the content of sulphated polysaccharides of: 661±2.30-733±1.55 mg/L which is 28.96-32.12 times respectively more than closest solution. The extracts possess superoxide dismutase activity of 54.68±2.82-38.41±0.42 U/mg protein, increased protein content 20.66±0.12-26.50±0.06% d.w. and carbohydrates 23.69±0.73-20.71±0.42% d.w. Applications: Agriculture, food industry and cosmetics. The research was carried out within the project 20.80009.5107.16 "New biologically active microbial preparations for increasing the reproductive and productive potential of animals of zootechnical interest", funded by NARD.