Antioxidant extracts based on natural pigments

Abstract

The invention relates to microbial biotechnologies, specifically to obtaining of new antioxidant extracts based on pigments from Arthrospira platensis cyanobacteria biomass, a residue from the production of the BioR remedy. The essence of the invention is the proposal of a new process for obtaining antioxidant extracts based on pigments from Arthrospira platensis cyanobacteria. The remaining biomass, dried at the temperature of +50±5°C, is ground, and mixed with 96% ethyl alcohol in the 1:10 v/v ratio. The suspension is supposed to sonication (50 W) for 5 minutes or placed in a water bath at the temperature of +45°C for 30 minutes with periodic stirring. At the end of the process, the extract is separated from the biomass by centrifugation at 3500 rpm. for 5 minutes. To obtain of the concentrated extract, the ethyl alcohol is removed using a rotary evaporator. The technical result of the invention consists in obtaining of the antioxidant ethanolic extracts based on natural pigments with a content of β-carotene of 0.645±0.001-0.6875±0.010 mg/100g, with 222-236% more than the closest solution. The extracts have catalase activity of 659.02±13.20-720.14±7.10 mmol/min/mg protein, superoxide dismutase activity of 107.52±20-545.95±1.16 U/mg protein and the concentrated extract contains 14.21±0.020 mg/100g of β-carotene, 0.569±0.001 mg/100g of lutein, 14.243±0.066 mg/l of chlorophyll a, 442.5±0.58 mg/l of sulfated polysaccharides, total antioxidant activity of 195.93±9.15% inhibition, catalase activity of 1235±30.59 mmol/min./mg protein, superoxide dismutase activity of 618±2.6 U/mg protein. This process can be used to obtain extracts based on pigments for use in the zootechnical sector, the food industry and cosmetics. The invention was developed within the project 20.80009.5107.16 "New biologically active microbial preparations for increasing the reproductive and productive potential of animals of zootechnical interest", funded by NARD.