Process for submerged cultivation of strain Lentinus edodes (BERK.) SING. CNMN-FB-01

Abstract

The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMNFB-01 fungi strain, producer of biomass. The method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain includes the inoculation of seed material in the amount of 10% v/v into a nutrient medium, containing, g/L: NH4NO3 – 0.20, KH2PO4 – 1.30, MgSO47H2O – 0.35, tris (2,6-dimethyl pyridinedicarboxylate-1kONO)-di-(isothiocyanato-1.2kN) - (diisocyanato-2kN) barium (II) cobalt (II) – 0.005-0.015, beer wort 5°Balling the rest, and cultivation with continuous stirring at a temperature of 28-30°C for 144 hours. The technical result of the invention consists in reducing the duration of cultivation by 48 hours and increasing the production of biomass by 35.7-38.2%. The results obtained can be conditioned by the enrichment of the nutrient medium with bivalent cations of the metals with a role biologically significant for the development of microorganisms (Co2+, Ba2+).