The influence of certain obstetric factors on the collection, isolation and quality of cord blood units

Abstract

The umbilical cord blood is a rich source of hematopoietic and mesenchymal stem cells and it can be used for cellular therapy. This article presents data taken from 50 cord blood samples (46 in utero, 4 ex utero). The nucleated cells were isolated from the cord blood using a dextran solution. The quantity of stem cells having the immunophenotype CD34+, CD45+ and CD90+ was determined, using flow cytometry. The nucleated cells were cultivated in a DMEM (DulbeccoModified Eagle Medium) and biochemical and cytogenetic research was later performed.