Process for extracting mannoproteins from brewer's yeast sediments

Abstract

Procedeul, conform invenţiei, prevede utilizarea biomasei de levuri din deşeurile industriei de bere (30 g) care se amestecă cu 30 ml soluţie tampon fosfat de sodiu (raport 1:1), apoi se supune autolizei la +37°C sau +45°C timp de 8 ore, cu agitare periodică, centrifugarea, prelucrarea sedimentelor cu soluţie 1N NaOH (raport 1:5) la temperatura de +80±5°C timp de 2 ore, apoi se efectuează centrifugarea la 3500 rot./min timp de 15 min şi supernatantele alcaline obţinute se sedimentează cu alcool etilic 96% în volum 1:2. Procedeul permite obţinerea cu 7-16% mai multe manoproteine comparativ cu varianta martor.

The process according to the invention includes the use of brewer's yeast biomass (30 g) which is mixed with 30 ml of sodium phosphate buffer (1: 1 ratio) then subjected to autolysis at +37°C or +45°C for hours, with periodic stirring, then centrifugation and the process of remaining the sediments with 1N NaOH (1:5) ratio solution at +80±5°C for 2 hours, centrifugation at 3500 rpm. For 15 minutes, the alkaline supernatants obtained were sedimented with 96% ethyl alcohol in a volume of 1:2. The process allows obtaining 7-16% more mannoproteins compared to the control variant.