The influence of high temperature on the protein fractions of bull semen

Abstract

Proteins are the most important organic substances which are part of the composition of living cells. Since the specific capabilities of spermatozoa depend on the protein, occurs the necessity of studying the characteristics of changes of the spectrum of the protein fractions of sperm depending on the ambient temperature. In this experiment was studied the effect of temperature of 45°C on the spectrum of protein fractions of the bull semen. From the data of the conducted research it is observed that when sperm of bull was exposed to a temperature of 45°C for 1 minute, has been a trend of increasing the quantity of albumin up to 4.2% and at 10 minutes - up to 3.3% of the total volume. When exposed to a temperature of 45°C for 1 minute the total amount of globulins, typically does not significantly change. The content of α-globulins tends to decrease, while the β- and γ-globulins do not support the changes. After 10 minutes of stress the amount of albumins is also increasing in comparison with the control sample and the quantity of total globulins decreased, especially α - and γ-globulins, while the content of β-globulin is not changed. Thus, the hyperthermic stress of bull semen for 1 minute is reflected only on the quantity of albumin, which has a tendency to increase. The influence of hyperthermic stress for 10 min is manifested by the tendency of growth of the percentage content of albumin and insignificant decrease in the concentration of total globulins, including α-globulin.