Crioconservarea spermei de vier

Abstract

The aim of the study was to improve the protocol for cryopreservation of boar semen. The studied biological material was obtained from boars by manual method. The sperm fraction, which was the richest in sperm, with a sperm motility of at least 70%, was taken for processing. After collection, the macroscopic and microscopic parameters of the collected sperm were assessed using the CEROS computer method. Semen dilution was performed using two dilution media. The first dilution was carried out with diluent A in a 1: 2 ratios at room temperature. The second dilution was carried out with diluent B by adding two parts of diluent at a temperature of + 2- + 4 ° C. The diluted and chilled semen was frozen at a temperature of -196 ° C. After resuscitation (thawing), sperm motility was 41.0 ± 0.6 % in the experimental group and 25.0 ± 1.7% in the control group. The difference was statistically significant (P≤0.01): spermatozoa with forward movements after resuscitation in the experimental group - 21.3 ± 2.8%, and in the control group 10 - ± 1.0, and the difference is significant (P≤0,1). The speed of movement of spermatozoa after VAP resuscitation in the control group was 57.2 ± 2.2 µm / sec, and in the experimental group it was 61.6 ± 4.4 µm / sec. After resuscitation, the VSL sperm advancement rate in the control group was 36.1 ± 1.3 μm / sec, and in the experimental group it was 41.2 ± 4.5 μm / sec. Changes also occurred in the VCL sperm rate, but the differences between the groups were not the true "genetics". Experimental results obtained after the implementation of the boar semen freezing protocol demonstrate the effectiveness of the proposed freezing method and can be used for collecting and storing frozen boar semen obtained from domestic and imported breeds with a valuable genetic fund in the "Genetic Bank".