Determination of tetracycline antibiotics in meat by the immunoenzymatic method

Abstract

In the present study, pork samples were examined for the detection of antibiotics from the tetracycline group by the immunoenzymatic method, using kits from the manufacturer R-biopharm and Biopanda Reagen. The antibiotics tetracycline, oxytetracycline and chlortetracycline were determined. The principle of the method involves the quantitative identification of the antibiotic present in the examined sample, where the fixed amount of antigen lined in the wells of the microtiter strips competes for anti-tetracycline, anti-oxytetracycline and anti-chlortetracycline antibodies which in turn are detected with the help of the enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualized by adding the buffer solution. The measurement is performed photometrically at a wavelength of 450 nm after the addition of the stop solution. The absorbance value depending on the color developed is inversely proportional to the amount of antibiotic in the sample. The amount of antibiotics in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.