Browsing 2020 by Author "MITINA, Irina"

Now showing items 1-3 of 3

Detection and quantification of mycotoxigenic fungi in maize by real-time PCR

MITINA, Irina; MITIN, Valentin; TUMANOVA, Lidia; ZGARDAN, Dan; STURZA, Rodica (Tehnica UTM, 2020)

The article focuses on real-time PCR detection and quantification of Fusarium spp., potential producer of class B fumonisin mycotoxins in mature corn kernels using primers designed to genes FUM1 and FUM6, involved in ...
Methodological aspects of real-time PCR usage in acetobacter detection

BOISTEAN, Alina; CHIRSANOVA, Aurica; ZGARDAN, Dan; MITINA, Irina; GAINA, Boris (Tehnica UTM, 2020)

Acetic acid bacteria occur in sugar and alcoholised, slightly acid niches such as flowers, fruits, beer, wine, cider, vinegar, souring fruit juices and honey. On these substrates they oxidize the sugars and alcohols, ...
Some aspects of primer design for real time PCR with SYBR® Green as a dye

MITIN, Valentin; MITINA, Irina (Tehnica UTM, 2020)

This work considers the sequence of steps for designing primers for real time qPCR analysis with SYBR® Green as fluorescent dye. For primer design, we used Primer-Blast software. As an example, we design primers to fum6 ...

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