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The methodological aspects of using real-time polymerase chain reaction (RT-PCR) in Brettanomyces/Dekkera detection

Show simple item record MITINA, Irina ZGARDAN, Dan STURZA, Rodica SCUTARU, Iurie 2019-11-03T14:26:20Z 2019-11-03T14:26:20Z 2019
dc.identifier.citation MITINA, Irina, ZGARDAN, Dan, STURZA, Rodica et al. The methodological aspects of using real-time polymerase chain reaction (RT-PCR) in Brettanomyces/Dekkera detection. In: Journal of Engineering Science. 2019, vol. 26(2), pp. 118-126. ISSN 2587-3474. eiSSN 2587-3482. en_US
dc.identifier.issn 2587-3474
dc.identifier.issn 2587-3482
dc.description.abstract Wine spoilage could be caused by a lot of genera and species of yeasts. One of the most damaging is Brettanomyces/Dekkera which causes severe quality problems in the wine industry. Timely detection of these organisms in wine is of crucial importance for preserving wine quality. The conventional methods of diagnostics are often time-consuming and laborious. For this, molecular- ased methods of rapid testing, particularly real-time PCR are gaining increasing importance. In this work, the potential of the real-time PCR method in microbiological monitoring of wines was evaluated, and analysis process according to the PIKA WeihenstephanTM SO Detection Kit H Brettanomyces/Dekkera protocol was optimized. en_US
dc.description.abstract Alterarea vinului poate fi cauzată de diferiţi agenţi patogeni, inclusiv de levuri sălbatice. Unul din genurile de levuri care cauzează probleme mari în industria vinicolă. Detecţia la timp a acestor microorganisme are un rol crucial în asigurarea unei calităţi superioare a vinurilor. Metodele moleculare de detecţie, ce se bazează pe amplificarea ADN-ului, inclusiv real-time PCR, sunt mai eficiente decât cele tradiţionale, deoarece acestea din urmă necesită mult timp și un volum mare de lucru. În această lucrare, s-a evaluat potenţialul metodei real-time PCR în monitorizarea microbiologică a vinurilor și s-a optimizat procesul de analiză în conformitate cu protocolul PIKA WeihenstephanTM SO Detection Kit H Brettanomyces/Dekkera. ro
dc.language.iso en en_US
dc.publisher Technical University of Moldova en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri *
dc.subject red wines en_US
dc.subject spoilage yeast en_US
dc.subject AND en_US
dc.subject deoxyribonucleic acid en_US
dc.subject Brettanomyces/Dekkera en_US
dc.subject PCR en_US
dc.subject Polymerase chain reaction en_US
dc.subject volatile phenols en_US
dc.subject cycle quantification en_US
dc.subject vinuri roşii en_US
dc.subject drojdii de degradare en_US
dc.subject en_US
dc.subject acid dezoxiribonucleic en_US
dc.subject fenoli volatili en_US
dc.subject cuantificare cicluri en_US
dc.title The methodological aspects of using real-time polymerase chain reaction (RT-PCR) in Brettanomyces/Dekkera detection en_US
dc.title.alternative Aspecte metodologice ale utilizării real-time polymerase chain reaction (RT-PCR) pentru detecţia Brettanomices / Dekkera en_US
dc.type Article en_US

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