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Screening-ul germoplasmei de floarea-soarelui la rugină

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dc.contributor.author DUCA, Maria
dc.contributor.author ŞESTACOVA, Tatiana
dc.contributor.author PORT, Angela
dc.contributor.author CUCEREAVÎI, Aliona
dc.contributor.author GISCA, Ion
dc.contributor.author TABĂRĂ, Olesea
dc.date.accessioned 2023-10-11T06:13:15Z
dc.date.available 2023-10-11T06:13:15Z
dc.date.issued 2014
dc.identifier.citation DUCA, Maria, ŞESTACOVA, Tatiana, PORT, Angela et al. Screening-ul germoplasmei de floarea-soarelui la rugină. In: Ştiinţa Agricolă. 2014, nr. 2, pp. 15-19. ISSN 1857-0003, eISSN 2587-3202. en_US
dc.identifier.issn 1857-0003
dc.identifier.issn 2587-3202
dc.identifier.uri http://repository.utm.md/handle/5014/24387
dc.description.abstract O caracteristică esenţială a hibrizilor comerciali de floarea-soarelui reprezintă rezistenţa la diferite boli şi dăunători, ceea ce exclude utilizarea tratamentelor chimice şi diminuează impactul negative asupra mediului. Rugina reprezintă o maladie micotică provocată de Puccinia helianthi ce cauzează pierderi semnificative de recoltă. Screeningul molecular al genelor de rezistenţă la rugină oferă posibilitatea identificării formelor parentale şi hibrizilor rezistenţi la infecţie. În acest studio s-au evaluat 42 genotipuri de floarea-soarelui (linii paterne Rf, linii materne cu androsterilitate citoplasmatică (ASC) şi hibrizi F1 comerciali) pentru a se stabili prezenţa ampliconului de 950 pb asociat cu gena R1, ce conferă rezistenţă la rasa de rugină 100. Analiza SCAR (SequenceCharacterized Amplified Regions) a demonstrat lipsa ampliconului de 950 pb la toate liniile materne, ceea ce indică faptul că aceste genotipuri nu posedă gena R1. Fragmentul de 950 pb a fost identificat doar la 13 genotipuri de floarea soarelui: 10 linii paterne şi 3 hibrizi comerciali autohtoni F1 (Nistru, Doina şi Oscar). Datele obţinute furnizează informaţii utile amelioratorilor privind selectarea combinaţiilor parentale în crearea hibrizilor rezistenţi la rugină. en_US
dc.description.abstract Akey feature of sunflower commercial hybridsis the resistance to various diseases and pests, which excludes the use of chemical treatments and reduces negative environmental impacts. Rust is a fungal disease caused by Puccinia helianthi leading to significant yield losses. The molecular screening of rust resistance genes offers the possibility of identifying parental forms and hybrids resistant to infection. In this study 42 sunflower genotypes (Rf paternal lines, maternal lines with cytoplasmic male sterility (CMS) and F1 commercial hybrids) were evaluated for the presence of the amplicon 950 pb associated with R1 gene, which confer resistance to the rust race 100. Molecular SCAR analysis (Sequence Characterized Amplified Regions) showed that the amplicon was not present at CMS lines indicating that these genotypes do not possess R1 gene. The fragment of 950 bp was found only in 13 genotypes: 10 paternal lines and three local commercial F1 hybrids (Nistru, Doina and Oscar). The data provide useful information to breeders for selecting parental combinations to create hybrids resistant to rust.
dc.language.iso ro en_US
dc.publisher Universitatea Agrară de Stat din Moldova en_US
dc.relation.ispartofseries Ştiinţa Agricolă;2014, N. 2
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject Helianthus annuus en_US
dc.subject screening en_US
dc.subject markeri genetici en_US
dc.subject rezistenţă la boli en_US
dc.subject rugină (fitopatologie) en_US
dc.subject screening en_US
dc.subject genetic markers en_US
dc.subject rusts en_US
dc.subject disease resistance en_US
dc.title Screening-ul germoplasmei de floarea-soarelui la rugină en_US
dc.title.alternative Screening sunflower germplasm for rust resistance en_US
dc.type Article en_US


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