Electrokinetic method for detection of DNA molecules

Abstract

In this study, we demonstrate the possibility of the Si-glass microchip capturing DNA samples by functionalizing the surface with PNA molecules as the essential bioreceptive element. Measurements are carried out based on electrokinetic principles, where the measuring element is the streaming current appearing on the surface through the flow of the liquid in a capillary. The obtained measurements demonstrate an optimal concentration of 31.25μM PLL-Thiol that covers the active surface of the microchip to the maximum that allows the capture of samples used continuously without loss. Furthermore, the capture of DNA is observed by the multiple changes of the signal by ~5mV, which allows us, based on the results, to continue the research to obtain a biosensor suitable for detecting genetic mutations.