Initiation of micropropagation of the fern – Osmunda regalis L.

Abstract

In vitro micropropagation is a modern and efficient technique for plant multiplication under aseptic laboratory conditions, enabling the production of large numbers of virus-free and pathogen-free microplants. This method is independent of climatic conditions and is carried out in controlled growth chambers with regulated airflow, optimal temperature and humidity, and appropriate lighting. Biotechnological approaches are widely applied for the conservation and propagation of rare and endangered species, including ferns. The growing demand for ornamental ferns highlights the need for propagation techniques that allow rapid production of large numbers of plants. The present study aimed to initiate and multiply in vitro the species Osmunda regalis L. within the Laboratory of Embryology and Biotechnology of “Al. Ciubotaru” National Botanical Garden (Institute).