Effect of dissolution media on the encapsulation efficiency of carotenoid-rich extract in liposomes

Abstract

This study aims to evaluate the impact of the dissolution media on encapsulation efficiency of sea buckthorn lipophilic extract rich in carotenoids in liposomes. Encapsulated bioactive compounds liposomes were prepared by an adapted heating method (Mozafari method) [1]. The study of liposomes incorporating lipophilic extracts from sea buckthorn reveals distinct encapsulation efficiencies, retention rates, and amounts of encapsulated bioactive compounds. The CLW sample exhibited the highest encapsulation efficiency (EE) at 90.90%, indicating superior encapsulation of carotenoids when water was used as the dissolution medium. Similar to the encapsulation efficiency, the water-based liposomes (CLW) demonstrated higher retention rates at 86.74%. The ethanol-based liposomes (CLEt) had slightly lower encapsulation efficiency of 87.83% and lower retention rates of 80.18%. The amount of encapsulated bioactive compounds (EBA) reflects the actual quantity of bioactives trapped within the liposomes. The CLW sample contained the highest amount of encapsulated bioactive compounds at 83.74 μg, followed closely by CLEt at 81.18 μg [2]. These results suggest that both water and ethanol can be used as dissolution media, but water tends to be slightly more effective in preserving higher amounts of encapsulated bioactives. The findings

indicate that liposomes prepared in double-distilled water generally outperform those prepared in ethanol in terms of encapsulation efficiency, retention rate, and the amount of encapsulated bioactive

compounds. The superior performance of water-based liposomes could be attributed to better stability and integrity of the liposomal bilayers in aqueous environments, which enhances the encapsulation and retention of bioactive compounds.