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Ascorbic acid determination from alcoholic and soft drinks by different techniques

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dc.contributor.author VARODI, C.
dc.contributor.author SORAN, M.-L.
dc.contributor.author LUNG, I.
dc.contributor.author OPRIȘ, O.
dc.contributor.author MUREȘAN, L.
dc.date.accessioned 2019-11-15T11:30:16Z
dc.date.available 2019-11-15T11:30:16Z
dc.date.issued 2016
dc.identifier.citation VARODI, C., SORAN, M.-L., LUNG, I. et al. Ascorbic acid determination from alcoholic and soft drinks by different techniques. In: Modern technologies, in the food industry–2016 (MTFI-2016): proc. of the intern. conf., 20-22 october, 2016. Chişinău, 2016, pp. 395-400. ISBN 978-9975-87-138-9. en_US
dc.identifier.isbn 978-9975-87-138-9
dc.identifier.uri http://repository.utm.md/handle/5014/6928
dc.description.abstract The present study consists in a comparative evaluation of the L-ascorbic acid (AA) concentration from some commercial Romanian juices and wines. The samples was analyzed by high-performance liquid chromatography (HPLC) on an Alltima C(18) column with isocratic elution of methanol and water with formic acid (pH 3) (65:35, v/v) at a flow rate of 0.3 mL/min, a column temperature of 26°C, and UV detection at 243 nm. The amperometric measurements were carried out under magnetic stirring using 0.1 M phosphate buffer solution as supporting electrolyte and pH 7 with a new biosensor, consisting in ascorbate oxidase (AOx) and bovine serum albumine immobilization on the membrane of an oxygen electrode, followed by cross-linking with glutaraldehyde (GA). Our study showed that the biosensor works well in synthetic samples of AA and may be recommended for testing in real samples (wine and fruit juices). en_US
dc.language.iso en en_US
dc.publisher Tehnica UTM en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject L-ascorbic acid en_US
dc.subject HPLC en_US
dc.subject electrochemical method en_US
dc.title Ascorbic acid determination from alcoholic and soft drinks by different techniques en_US
dc.type Article en_US


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