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Protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles

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dc.contributor.author CIOBANU, Vladimir
dc.contributor.author RONCARI, Francesco
dc.contributor.author CECCONE, Giacomo
dc.contributor.author BRANISTE, Tudor
dc.contributor.author PONTI, Jessica
dc.contributor.author BOGNI, Alessia
dc.contributor.author GUERRINI, Giuditta
dc.contributor.author CASSANO, Domenico
dc.contributor.author COLPO, Pascal
dc.contributor.author TIGINYANU, Ion
dc.date.accessioned 2025-02-19T08:20:37Z
dc.date.available 2025-02-19T08:20:37Z
dc.date.issued 2022
dc.identifier.citation CIOBANU, Vladimir; Francesco RONCARI; Giacomo CECCONE; Tudor BRANISTE; Jessica PONTI; Alessia BOGNI; Giuditta GUERRINI; Domenico CASSANO; Pascal COLPO and Ion TIGINYANU. Protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles. Journal of Applied Biomaterials and Functional Materials. 2022, vol. 20. ISSN 2280-8000. en_US
dc.identifier.issn 2280-8000
dc.identifier.uri https://doi.org/10.1177/22808000221131881
dc.identifier.uri https://repository.utm.md/handle/5014/29687
dc.description Access full text: https://doi.org/10.1177/22808000221131881 en_US
dc.description.abstract The interaction of semiconductor nanoparticles with bio-molecules attracts increasing interest of researchers, considering the reactivity of nanoparticles and the possibility to control their properties remotely giving mechanical, thermal, or electrical stimulus to the surrounding bio-environment. This work reports on a systematic comparative study of the protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles. Bovine serum albumin was chosen as a protein model. Dynamic light scattering, transmission electron microscopy and X-ray photoelectron spectroscopy techniques have been used to demonstrate the formation of protein-corona as well as the stability of the colloidal suspension given by BSA, which also works as a surfactant. The protein adsorption on the NPs surface studied by Bradford Assay showed the dependence on the quantity of proteins adsorbed to the available sites on the NPs surface, thus the saturation was observed at ratio higher than 5:1 (NPs:Proteins) in case of ZnO, these correlating with DLS results. Moreover, the kinetics of the proteins showed a relatively fast adsorption on the NPs surface with a saturation curve after about 25 min. GaN NPs, however, showed a very small amount of proteins adsorbed on the surface, a change in the hydrodynamic size being not observable with DLS technique or differential centrifugal sedimentation. The Circular Dichroism analysis suggests a drastic structural change in the secondary structure of the BSA after attaching on the NPs surface. The ZnO nanoparticles adsorb a protein-corona, which does not protect them against dissolution, and in consequence, the material proved to be highly toxic for Human keratinocyte cell line (HaCaT) at concentration above 25 µg/mL. In contrast, the GaN nanoparticles which do not adsorb a protein-corona, show no toxicity signs for HaCaT cells at concentration as high as 50 µg/mL, exhibiting much lower concentration of ions leakage in the culture medium as compared to ZnO nanoparticles. en_US
dc.language.iso en en_US
dc.publisher SAGE Publications en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject protein-corona en_US
dc.subject nanoparticles en_US
dc.subject cell viability en_US
dc.title Protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles en_US
dc.type Article en_US


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